TUFM antibody | knockout validation | Thermo Fisher Scientific MA5-31363

This is a knockout-validated antibody summary, based on the publication "Inhibition of mitochondrial translation suppresses glioblastoma stem cell growth", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TUFM antibody | knockout validation | Thermo Fisher Scientific MA5-31363 figure 1
Figure 1. Effects of TUFM knockout on TUFM and β-tubulin proteins in COMI and VIPI cells, as assayed by immunoblotting. From [1].
Antibody information

Mouse monoclonal IgG1

Company: Thermo Fisher Scientific

Antibody: TUFM

Catalog number: MA5-31363

Summary: Mouse monoclonal antibody against a recombinant protein corresponding to human TUFM. Reacts with human by western blot, immunohistochemistry (paraffin) and immunocytochemistry/immunofluorescence.

Validation Method

Western blot

Sample

Control and TUFM knockout COMI and VIPI cell lines (human glioblastoma stem cells).

Primary incubation

1:1,000 dilution.

Secondary incubation

1:3,000 dilution goat anti-Mouse IgG - Horseradish Peroxidase conjugated (Santa Cruz Biotech, sc-2005).

Detection

Amersham ECL Prime or Select Western Blotting Detection Reagent (GE Healthcare Life Sciences) and ChemiDoc Imaging System (Bio-Rad).

Clone note

The same clone (CL2242) is sold as Novus Biologicals NBP2-36751; MilliporeSigma AMAB90964; Abnova MAB15734; Invitrogen MA5-31363.

References
  1. Sighel D, Notarangelo M, Aibara S, Re A, Ricci G, Guida M, et al. Inhibition of mitochondrial translation suppresses glioblastoma stem cell growth. Cell Rep. 2021;35:109024 pubmed publisher