This is a knockout-validated antibody summary, based on the publications "Translocator Protein (TSPO) Affects Mitochondrial Fatty Acid Oxidation in Steroidogenic Cells", as cited below [1], "CRISPR-Cas9 Mediated TSPO Gene Knockout alters Respiration and Cellular Metabolism in Human Primary Microglia Cells" for immunocytochemistry knockout validation (see [2] figure 1e), "Cholesterol-binding translocator protein TSPO regulates steatosis and bile acid synthesis in nonalcoholic fatty liver disease" for immunocytochemistry knockout validation (figure s11h) [3], "The translocator protein (TSPO) is prodromal to mitophagy loss in neurotoxicity" for western blot knockout validation (figure 5e) [4], "TSPO Deficiency Exacerbates GSDMD-Mediated Macrophage Pyroptosis in Inflammatory Bowel Disease" for (figure 4a,5a) [5], and "Mitochondrial translocator protein deficiency exacerbates pathology in acute experimental ulcerative colitis" for (figure 1a,1b) [6]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Abcam

Antibody: TSPO

Catalog number: ab109497

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human PBR aa 150 to the C-terminus. Reacts with human and mouse. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin) immunocytochemistry/immunofluorescence and flow cytometry.

Western blot

Tspo+/+ and TspoΔ/Δ MA-10 Leydig cells.

IRDye 700 and 800-labeled secondary antibodies.

Laser fluorescence scanner (Li-Cor).

Please see Figure 1a in the article [1].