This is a knockout-validated antibody summary, based on the publication "TRPV2 is critical for the maintenance of cardiac structure and function in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: Millipore

Antibody: TRPV2

Catalog number: AB5398

Summary: Rabbit polyclonal against a peptide corresponding to the C-terminal 18 amino acids of the rat VRL-1 protein sequence. Reacts with rat protein by immunohistochemistry.

Western blot | Immunohistochemistry

Heart and kidney from tamoxifen-treated and -untreated TRPV2flox/flox or TRPV2flox/flox;MerCreMer+/− (TRPV2flox/flox; Cre+/−) mice. For WB: homogenized in a Hiscotron homogenizer (NITI-ON) in lysis buffer containing 20 mM HEPES (pH 7.4), 150 mM NaCl, 1% sodium deoxycholate, 1% SDS, 2 μg ml−1 leupeptin, 1 μg ml−1 aprotinin, 200 μM phenylmethylsulfonyl fluoride, and 200 μM benzamidine hydrochloride. For IHC: 5 μm frozen heart sections embedded in OCT compound (Tissue-Tek) were permeabilized with 0.1% Triton X-100 and incubated with primary antibodies.

1:200 dilution.

WB: chemiluminescence detection system (Perkin Elmer) and an LAS3000 Luminescent Image Analyzer (Fuji Film).

IHC: confocal microscope (Fluoview FV1000, Olympus) mounted on an Olympus IX81 epifluorescence microscope with a UPlanSApo × 60/1.35 oil immersion objective lens (Olympus).

Please see Figure 1d,e in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.