TRPM6 antibody | knockout validation | Santa Cruz sc-365536

This is a knockout-validated antibody summary, based on the publication "Histone phosphorylation by TRPM6's cleaved kinase attenuates adjacent arginine methylation to regulate gene expression", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TRPM6 antibody | knockout validation | Santa Cruz sc-365536 figure 1
Figure 1. Immunoblot of TRPM6 in 293T parental (WT) and TRPM6−/− clonal (KO25) cells immunoprecipitated from cell lysates with TRPM6 C-terminal rabbit antibody (αM6C14) and probed with mouse TRPM6 antibody (Santa Cruz, sc-365536). Courtesy from the Authors.
Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: TRPM6

Catalog number: sc-365536

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1225-1524 mapping within an internal region of human TRPM6. Recommended for detection of TRPM6 of human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT and TRPM6−/− 293T cells.

References
  1. Krapivinsky G, Krapivinsky L, Renthal N, Santa-Cruz A, Manasian Y, Clapham D. Histone phosphorylation by TRPM6's cleaved kinase attenuates adjacent arginine methylation to regulate gene expression. Proc Natl Acad Sci U S A. 2017;114:E7092-E7100 pubmed publisher