This is a knockout-validated antibody summary, based on the publication "An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. (Upper panel) Western blot analysis of each lymphoma for TRP53 protein and p19 with probing for b-actin used as a loading control. 41.2% of Eμ-Myc isgTrp53 tumors assessed display abnormally high (stabilized) TRP53 protein, indicative of mutations in Trp53. (Lower panel) Western blot analysis of lymphomas before and after treatment with etoposide (0.5 mg/ml for 24 hr) in the presence of the broad-spectrum caspase inhibitor QVD-OPH (25 mM) to prevent apoptosis. i: inducible, cont: constitutive. From [1].

Rabbit polyclonal IgG

Company: Novocastra

Antibody: TRP53

Catalog number: NCL-p53-CM5p

Summary: Rabbit polyclonal IgG against recombinant mouse p53 protein. Reacts with mouse and rat. Suitable for western blot and immunohistochemistry (paraffin).

Western blot

Tumors formed by injection of Eμ-Myc lymphoma cells having WT, mutated or deleted p53. Cells and primary tumor samples were lysed in RIPA buffer supplemented with protease inhibitors (complete protease inhibitor cocktail, Roche).

5% milk in PBS with 0.1% Tween 20.