This is a knockout-validated antibody summary, based on the publication "One-step generation of conditional and reversible gene knockouts", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: TRIM37
Catalog number: sc-514828
Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 152-451 mapping within an internal region of human TRIM37. Recommended for detection of TRIM37 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.
Western blot
Control and TRIM37-KO mouse embryonic stem cells (mESC). Cells were lysed in buffer containing complete protease-inhibitor cocktail tablets (11697498001, Roche).
1:500 dilution.
1:5,000 dilution horse anti-mouse IgG HRP conjugated (Cell Signaling).
ECL prime western blotting detection system (RPN2133, GE Healthcare).
Loss of TRIM37 protein expression following Cre recombination was confirmed by western blot (q). Please see Supplementary Figures 7q in the article [1].
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