This is a knockout-validated antibody summary, based on the publication "Acetylation Blocks cGAS Activity and Inhibits Self-DNA-Induced Autoimmunity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2a (kappa light chain)
Company: Santa Cruz
Antibody: TREX-1
Catalog number: sc-271870
Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 290-369 mapping at the C-terminus of human TREX-1. Recommended for detection of three prime repair exonuclease 1 of human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.
Western blot
Spleen of wild-type or Trex1−/− mice. Spleens of mice were homogenized with handheld homogenizer (PT 1200 E, Kinematica) and lysed in RIPA buffer (50 mM Tris-HCl pH 7.5, 1% Nonidet P-40, 150 mM NaCl, 5 mM EDTA, 0.5% Deoxycholate Sodium) supplemented with complete protease inhibitor cocktail, 1 mM TSA and 10 mM NAM.
Enhanced chemiluminescence according to the manufacturer’s instructions (ThermoFisher Scientific).
Immunoblot of the indicated proteins in spleen of wild-type or Trex1−/− mice. Anti-a-tubulin blots indicate loading of lanes. * indicates non-specific bands. WCL, whole cell lysate. Please see Suppl. Figure 6h in the article [1].
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