This is a knockout-validated antibody summary, based on the publication "Spadin Modulates Astrocytic Passive Conductance via Inhibition of TWIK-1/TREK-1 Heterodimeric Channels", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunocytochemical images of control gRNA (Con gRNA) or TREK-1 gRNA transfected cultured astrocytes stained with anti-TREK-1 antibody. From [1].

Mouse monoclonal IgM κ

Company: Santa Cruz

Antibody: TREK-1

Catalog number: sc-398449

Summary: Mouse monoclonal antibody specific for an epitope mapping between amino acids 354-380 near the C-terminus of human TREK-1. Recommended for detection of TREK-1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Immunocytochemistry

Control and TREK-1 KO cultured astrocytes.

0.1% Triton X-100 and 3% BSA for 1 h.

1:100 dilution at 4 °C overnight.

1:400 dilution Alexa Fluor 594-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, USA) for 1 h at room temperature.

Confocal microscopy using a Nikon A1 confocal microscope (Nikon Instruments Inc., Melville, NY, USA).