This is a knockout-validated antibody summary, based on the publication "Two-Pore K+ Channel TREK-1 Regulates Sinoatrial Node Membrane Excitability", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblots for TREK‐1 in heart, brain, and pancreas lysates from WT and αMHC‐Kcnk2 f/f mice (Kcnk2 −/−). TREK‐1 was first immunoprecipitated using an antibody against c‐terminal epitope (H‐75; Santa Cruz Biotechnology), and then immunoblot was performed using antibody against N‐terminal epitope (N‐20; Santa Cruz Biotechnology). From [1].

Goat polyclonal IgG

Company: Santa Cruz Biotechnology

Antibody: TREK-1

Catalog number: sc-11554

Summary: Goat polyclonal IgG against an epitope mapping at the N-terminus of TREK-1 of human origin. Recommended for detection of TREK-1 of mouse, rat, and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including and equine, canine, bovine, porcine and avian.

Western blot

Heart, brain, and pancreas lysates from WT and αMHC‐Kcnk2 f/f mice (Kcnk2 −/−).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.