This is a knockout-validated antibody summary, based on the publication "Hepatocyte TRAF3 promotes liver steatosis and systemic insulin resistance through targeting TAK1-dependent signalling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of TRAF3 protein levels in primary hepatocytes isolated from TRAF3+/+ and TRAF3−/− mice. From [1].

Rabbit polyclonal

Company: Cell Signaling

Antibody: TRAF3

Catalog number: 4729

Summary: Rabbit polyclonal against a synthetic peptide corresponding to a central region within TRAF3. Reacts with human, mouse, rat, and monkey protein by western blot.

Western blot

Primary hepatocytes isolated from TRAF3+/+ and TRAF3−/− mice. Cells were lysed using RIPA lysis buffer (720 μl of RIPA, 20 μl of phenylmethyl sulphonyl fluoride, 100 μl of complete protease inhibitor cocktail, 100 μl of Phos-stop, 50 μl of NaF and 10 μl of Na3VO4 in a final volume of 1 ml).

5% skim milk.

1:1,000 dilution at 4 °C overnight.

ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.