This is a knockout-validated antibody summary, based on the publication "A novel loss-of-function mutation in TP53 in an endometrial cancer cell line and uterine papillary serous carcinoma model", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1k
Company: Zymed (Thermo Fisher Scientific)
Antibody: TP53
Catalog number: 13-4100 (Clone PAB240)
Summary: Mouse monoclonal antibody raised against a recombinant p53 peptide. Supplier recommended for WB and IHC.
Western blot
Lysates of Hec50co TP53 null cells plus/minus transduction with TP53-expressing adenovirus.
HRP-conjugated anti-mouse IgG (Bio-Rad).
ECL +Plus reagent (Amersham).
See figure 5 in [1].
- Liu Z, Wan G, Heaphy C, Bisoffi M, Griffith J, Hu C. A novel loss-of-function mutation in TP53 in an endometrial cancer cell line and uterine papillary serous carcinoma model. Mol Cell Biochem. 2007;297:179-87 pubmed