This is a knockout-validated antibody summary, based on the publication "A20 Ameliorates Intracerebral Hemorrhage-Induced Inflammatory Injury by Regulating TRAF6 Polyubiquitination" [1] (discussed below) and "A20 Ameliorates Intracerebral Hemorrhage-Induced Inflammatory Injury by Regulating TRAF6 Polyubiquitination" [1] (see figure 6b in the article). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1

Company: Abcam

Antibody: TNFAIP3

Catalog number: ab13597

Summary: Mouse monoclonal IgG1 against recombinant fusion protein of full length human TNFAIP3. Reacts with: Mouse, Rat, Human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

Perihematomal brain tissues from WT, A20-/-, LV-A20 and LV-GFP mice. Tissues were lysed in RIPA buffer.

5% skim milk.

1:200 dilution overnight at at 4˚C.

HRP-conjugated goat anti-rabbit secondary Abs (1:200; Millipore) at 25˚C for 1.5 h.

Chemiluminescence detection system.

Protein lysates from WT, A20-/-, LV-A20 and LV-GFP groups at 1, 3, and 7 days after intracerebral hemorrhage (ICH) were subjected to immunoblotting with antibodies to A20 and GAPDH (loading control). Please see Figure 6b in the article [1].

The same clone (59A426) is sold as Novus Biologicals NBP1-77533, NBP1-77533AF405, NBP1-77533AF488, NBP1-77533AF647, NBP1-77533AF700, NBP1-77533APC, NBP1-77533B, NBP1-77533C, NBP1-77533FR, NBP1-77533G, NBP1-77533H, NBP1-77533IR, NBP1-77533PCP, NBP1-77533PE, NBP1-77533R, NBP1-77533UV, NBP1-77533V, NBP2-33252; OriGene TA336834; Santa Cruz Biotechnology sc-52910; LifeSpan Biosciences LS-C531, LS-C368301; Abcam ab13597; Rockland Immunochemicals 200-301-H52; Invitrogen MA5-16164.