TNF alpha antibody | knockout validation | Abcam ab1793

This is a knockout-validated antibody summary, based on the publication "Tumor necrosis factor alpha mediates neuromuscular synapse elimination", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TNF alpha antibody | knockout validation | Abcam ab1793 figure 1
Figure 1. Immunoblotting of the indicated proteins in TNF knockout mice. From [1].
Antibody information

Mouse monoclonal IgG1

Company: Abcam

Antibody: TNF alpha

Catalog number: ab1793

Summary: Mouse monoclonal IgG1 against full length human TNF alpha (purified native protein). Reacts with mouse, guinea pig, human, chimpanzee, zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus. Suitable for flow cytometry and immunocytochemistry/immunofluorescence.

Validation Method

Western blot

Sample

WT and TNF knockout mice limb muscle.

Clone note

The same clone (52B83) is sold as Novus Biologicals NB600-1422; Santa Cruz Biotechnology sc-52746; LifeSpan Biosciences LS-B2123, LS-C122512, LS-C139943, LS-C146598; Abcam ab1793; Hycult Biotech HM2010, HM2010F.

References
  1. Fu X, Peng J, Wang A, Luo Z. Tumor necrosis factor alpha mediates neuromuscular synapse elimination. Cell Discov. 2020;6:9 pubmed publisher