This is a knockout-validated antibody summary, based on the publication "Kinase-independent functions of RIPK1 regulate hepatocyte survival and liver carcinogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: TNF-R1
Catalog number: 13377
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy terminus of mouse TNF-R1 protein. Reacts with mouse and rat. Suitable for western blot.
Western blot
Liver tissue from 8-week-old control and Ripk1fl/fl Relafl/fl Tnfr1fl/fl Alfp-Cre mice . Tissues were homogenized using Precellys in protein lysis buffer supplemented with protease and phosphatase inhibitors (1 tablet per 10 ml lysis buffer, Roche).
5% milk/0.1% PBST.
For 16 hours at 4°C.
Horseradish peroxidase–conjugated anti-rabbit (GE Healthcare).
Enhanced chemiluminescent detection substrate (GE Healthcare and Thermo Scientific).
Immunoblot analysis of liver lysates from 8-week-old mice with the indicated genotypes. Actin was used as loading control. Please see Figure 2b in the article [1].
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