This is a knockout-validated antibody summary, based on publications "A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses", as cited below [1], and "Paeniclostridium sordellii hemorrhagic toxin targets TMPRSS2 to induce colonic epithelial lesions" for western blot knockout validation (figure 2d) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunohistochemical staining of TMPRSS2 in wildtype and TMPRSS2-knock out (KO) organoids. TMPRSS2 locates mostly to the apical membrane in wildtype cells, and is absent in mutant organoids. Scale bars are 50 µm. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: TMPRSS2

Catalog number: ab109131

Summary: Rabbit monoclonal antibody against a synthetic peptide from human TMPRSS2. Reacts with human. Suitable for western blot, immunohistochemistry (paraffin). Unsuitable for immunocytochemistry/immunofluorescence.

Immunohistochemistry

WT and TMPRSS2-KO human intestinal organoids.

1:100 dilution.

1:2,000 dilution goat-anti-rabbit conjugated to horseradish peroxidase (Dako, P0448).

Visualized with Powervision (Leica).