This is a knockout-validated antibody summary, based on the publication "VMP1, a novel prognostic biomarker, contributes to glioma development by regulating autophagy", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Schematic diagram illustrating the CRISPR-Cas9-mediated VMP1 editing system and western blotting to confirm the decrease in VMP1 expression. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: TMEM49/VMP1

Catalog number: 12978

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the amino terminus of human TMEM49/VMP1 protein. Reacts with human by western blot and immunoprecipitation.

Western blot

WT and VMP1-KO LN299 cell lines.

5% bovine serum albumin (cat #ST023, Beyotime Biotechnology) in Tris-buffered saline with Tween-20 (TBST) for 1 h at room temperature.

1:2,000 dilution at 4 °C overnight.

1:3,000 dilution HRP-conjugated antibody (cat #7074S, Cell Signaling Technology) for 2h.

Bio-Rad system (Hercules, CA, USA).