TMEM49/VMP1 antibody | knockout validation | Cell Signaling 12929

This is a knockout-validated antibody summary, based on the publication "TMEM41B Is a Pan-flavivirus Host Factor", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TMEM49/VMP1 antibody | knockout validation | Cell Signaling 12929 figure 1
Figure 1. Western blots for TMEM41A, TMEM41B, and VMP1 in KO clones lacking individual VTT domain-containing proteins. The expected sizes are indicated by labels on the right. β-actin is shown as loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: TMEM49/VMP1

Catalog number: 12929

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the carboxy terminus of human TMEM49/VMP1 protein. Reacts with human, mouse, and rat. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and VMP1 KO HAP1 cells.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:5,000 dilution goat anti-rabbit IgG conjugated to HRP (ThermoFisher Scientific, 31462).

References
  1. Hoffmann H, Schneider W, Rozen Gagnon K, Miles L, Schuster F, Razooky B, et al. TMEM41B Is a Pan-flavivirus Host Factor. Cell. 2021;184:133-148.e20 pubmed publisher