This is a knockout-validated antibody summary, based on the publication "Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of TLR2 protein in Sertoli and Leydig cells isolated from three-week-old WT and TLR2−/− mice. Beta-actin was used as loading control. From [1].

Antibody: TLR2

Catalog number: ab109810

Summary: Rat monoclonal raised against a synthetic peptide within amino acids 400-450 of mouse TLR2. Reacts with mouse protein and has been used in western blot and flow cytometry.

Company: Abcam

Western blot

Sertoli and Leydig cells were isolated from three-week-old WT and TLR2−/− mice. Cells were lysed with a lysis buffer (Applygen Technologies Inc., Beijing, China),

Tris-buffered saline (TBS, pH 7.4) containing 5% non-fat milk at room temperature for 1 h.

1:1000, overnight at 4 C

horseradish-peroxidase (HRP)-conjugated secondary antibodies at room temperature for 1 h.

enhanced chemiluminescence detection kit (Zhongshan Biotechnology Co.).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.