TLE3 antibody | knockout validation

TLE3 antibody | knockout validation | Santa Cruz sc-514798

This is a knockout-validated antibody summary, based on the publication "TLE3 loss confers AR inhibitor resistance by facilitating GR-mediated human prostate cancer cell growth", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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TLE3 antibody | knockout validation | Santa Cruz sc-514798 figure 1

Figure 1. Western blot showing TLE3 protein levels for control cells and TLE3KO cells. Vinculin was used as a loading control. From [1].

Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: TLE3

Catalog number: sc-514798

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 200-400 of mouse TLE3. Recommended for detection of TLE3 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

Control and TLE3-KO LNCaP cells.

Primary incubation

1:250 dilution

Secondary incubation

Secondary antibodies were obtained from Bio-Rad laboratories.

References

Palit S, Vis D, Stelloo S, Lieftink C, Prekovic S, Bekers E, et al. TLE3 loss confers AR inhibitor resistance by facilitating GR-mediated human prostate cancer cell growth. elife. 2019;8: pubmed publisher

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