TIM-1/KIM-1/HAVCR antibody | knockout validation | R&D Systems MAB1750

This is a knockout-validated antibody summary, based on the publication "TIM-1 Promotes Hepatitis C Virus Cell Attachment and Infection", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG2B

Company: R&D Systems

Antibody: TIM-1/KIM-1/HAVCR

Catalog number: MAB1750

Summary: Mouse monoclonal IgG2B against recombinant human TIM‑1/KIM-1/HAVCR (Ser21-Thr288). Reacts with human by western blot, flow cytometry and immunohistochemistry.

Validation Method

Western blot

Sample

Parental and TIM-1 knockout Huh-7.5 cells.

Blocking agent

5% nonfat milk.

Secondary incubation

Horseradish peroxidase (HRP)-conjugated anti-mouse.

Detection

Enhanced chemiluminescence (ECL) kit (Pierce) and a Chemi-Doc MP imaging system (Bio-Rad).

Figure

Validation of TIM-1 knockout by western blotting using a TIM-1-specific monoclonal antibody. Please see Figure 2b in the article [1].

Clone note

The same clone (219211) is sold as R & D Systems FAB1750G, FAB1750P, MAB1750-100, FAB1750N-100UG, FAB1750R-100UG, FAB1750S-100UG, FAB1750T-100UG, FAB1750U-100UG, FAB1750V-100UG; Invitrogen MA5-23614, MA5-23634.

References
  1. Wang J, Qiao L, Hou Z, Luo G. TIM-1 Promotes Hepatitis C Virus Cell Attachment and Infection. J Virol. 2017;91: pubmed publisher