TIF1 antibody | knockout validation | Millipore MAB3662

This is a knockout-validated antibody summary, based on the publication "KAP1 regulates endogenous retroviruses in adult human cells and contributes to innate immune control", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TIF1 antibody | knockout validation | Millipore MAB3662 figure 1
Figure 1. Western blot of KAP1 of WT and KAP1-KO HeLa (upper panel) and 293T (lower panel) cell clones. From [1].
Antibody information

Mouse monoclonal IgG2b

Company: Millipore

Antibody: TIF1

Catalog number: MAB3662

Summary: Mouse monoclonal IgG2b against recombinant protein from mouse TIF1b (a. a. 123-834). Reacts with human and mouse. Suitable for western blot, immunocytochemistry and ELISA.

Validation Method

Western blot

Sample

WT and KAP1-KO HeLa and 293T cell clones. Cells were lysed in NuPAGE LDS

sample buffer (Thermo Fisher) with 5% b-mercaptoethanol.

Secondary incubation

Horseradish peroxidase-conjugated (GE healthcare).

Detection

ECL kits (ECL, Prime or Select kits from Amersham).

References
  1. Tie C, Fernandes L, Conde L, Robbez Masson L, Sumner R, PEACOCK T, et al. KAP1 regulates endogenous retroviruses in adult human cells and contributes to innate immune control. EMBO Rep. 2018;19: pubmed publisher