This is a knockout-validated antibody summary, based on the publication "Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: TH-POK
Catalog number: sc-376250
Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 252-358 mapping within an internal region of human TH-POK (ZBTB7B). Recommended for detection of TH-POK of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.
Western blot
Brown fat lysates from control WT and Zbtb7b-knockout mice. Tissue lysates were prepared by homogenizing in a buffer containing 50 mM Tris (pH 7.6), 130 mM NaCl, 5 mM NaF, 25 mM β-glycerophosphate, 1 mM sodium orthovanadate, 10% glycerol, 1% Triton X-100, 1 mM DTT, 1 mM PMSF, and the protease inhibitor mixture.
Immunoblots of the indicated protein in brown fat lysates from control WT and Zbtb7b-knockout (ZKO) mice. Please see Figure 2b in the article [1].
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