This is a knockout-validated antibody summary, based on the publication "Transglutaminase type 2-dependent selective recruitment of proteins into exosomes under stressful cellular conditions", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of TG2 (TGM2) and tubulin (loading control) in the indicated cells. Courtesy from the Authors [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: TGM2

Catalog number: 3557

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Lys598 of human TGM2. Reacts with human, mouse, rat and monkey. Suitable for western blot, immunohistochemistry (paraffin) and immunofluorescence/immunocytochemistry.

Western blot

Exosomes from lysis buffer containing 20 mM Tris-HCl pH 7.4, 150 mM NaCl and 1% Triton X-100, with protease inhibitor cocktail (Roche).

5% non-fat dry milk in T-PBS (PBS + 0.05% Tween 20) for 1 h at room temperature.

Overnight.

HRP-conjugated goat anti-rabbit IgG (H + L) (170–6515, Bio-Rad Laboratories) for 1 h at room temperature.

Immun-Star WesternC Kit (Bio-Rad Laboratories).