This is a knockout-validated antibody summary, based on the publication "A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b, kappa light chain
Company: DSHB
Antibody: TFAP2A
Catalog number: 3B5
Summary: Mouse monoclonal antibody against AP-2 alpha delta N165 (epitope sequence: amino acids 166-197). Reacts with chicken, human and mouse. Suitable for western blot, immunofluorescence, immunohistochemistry, chromatin immunoprecipitation (ChIP), and gel supershift (EMSA).
Immunocytochemistry
Wild-type and TFAP2A KO hESCs.
AlexaFluor Donkey Anti-Mouse (Thermo Fisher Scientific, A-31570 or A-21235).
Immunofluorescence of TFAP2A in wild-type and TFAP2A KO differentiating cells treated with BMP4 for 3 days. Please see Figure 5a in the article [1].
The same clone (3B5) is sold as Novus Biologicals NB100-74359; Santa Cruz Biotechnology sc-12726, sc-12726 X; Invitrogen MA1-872; Developmental Studies Hybridoma Bank 3B5.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.