This is a knockout-validated antibody summary, based on the publication "Tead1 Is Required for Perinatal Cardiomyocyte Proliferation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunostaining in day 1 heart section verifying Tead1 deletion. Scale bar, 50μm. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: TEF1/TEAD-1

Catalog number: ab133533

Summary: Rabbit monoclonal IgG against a synthetic peptide within Human TEF1/TEAD-1 (aa 200-300). Reacts with mouse, rat, and human. Suitable for western blot, immunohistochemistry (paraffin) and immunoprecipitation. Unsuitable for flow cytometry or immunocytochemistry.

Western blot | Immunohistochemistry

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Figure 2. Western blot validation for Tead1 knockout in stable HL-1 cells. From [1].

WB: Tissue protein samples were prepared in ice-cold lysis buffer (100 mmol/L TrisHCl pH7.4, 10 mmol/L NaCl, 1 mmol/L EDTA, 1 mmol/L EGTA, 10% glycerol, 0.5% deoxycholate, 1% Triton X-100, 0.1% SDS, 20mmol/L Na4P2O7, 2 mmol/L Na3VO4, 1 mmol/L NaF, 1 mmol/L PMSF) supplemented with 1X protease inhibitor and phosphatase inhibitor (Roche). IHC: antigen retrieval in tissue sections in boiling 10mmol/L sodium citrate (pH 6.0) for 30min.

WB: 5% non-fat milk-TBS (w/v). IHC: 10% goat serum.

WB: 1:2,000 dilution. IHC: 1:100 dilution.

WB: HRP-conjugated secondary antibodies. IHC: 1:200 dilution Alexa Fluor conjugated anti-mouse IgG (Invitrogen).

WB: enhanced chemiluminescence substrate (Thermo Sci.). IHC: DeltaVision (Deconvolution) image restoration microscope or a Zeiss Axioplan-2 imaging system.