TCF4/TCF7L2 antibody | knockout validation | Cell Signaling 2565

This is a knockout-validated antibody summary, based on the publication "β-catenin and γ-catenin are dispensable for T lymphocytes and AML leukemic stem cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TCF4/TCF7L2 antibody | knockout validation | Cell Signaling 2565 figure 1
Figure 1. In vivo ablation of Tcf/Lef proteins. CreER+ WT, Tcf1/Lef1 dKO, and Tcf3/4 dKO mice were treated with tamoxifen for four consecutive days. Total thymocytes were immunoblotted for Tcf4 protein in Tcf3/4 and control mice. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: TCF4/TCF7L2

Catalog number: 2565

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Glu81 of human TCF4/TCF7L2. Reacts with human and it is predicted to react based on 100% sequence homology with mouse and rat. Suitable for western blot, immunoprecipitation (IP) and Chromatin IP.

Validation Method

Western blot

Sample

Control and Tcf3/4-DKO mouse thymocytes.

References
  1. Zhao X, Shao P, Gai K, Li F, Shan Q, Xue H. β-catenin and γ-catenin are dispensable for T lymphocytes and AML leukemic stem cells. elife. 2020;9: pubmed publisher