TCF11/NRF1 antibody | knockout validation | Cell Signaling 8052
This is a knockout-validated antibody summary, based on the publication "A Homeostatic Shift Facilitates Endoplasmic Reticulum Proteostasis through Transcriptional Integration of Proteostatic Stress Response Pathways", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Figure 1. Immunoblot of NRF1 in WT and Nrf1-KO MEFs. In response to the proteasome inhibitor MG132, NRF1 protein significantly accumulates within cells. Two isoforms of NRF1 are present in MEFs, a high molecular weight form, and a cleaved low molecular weight nuclear form. Tubulin was used as loading control. Courtesy of the authors. The first 3 lanes are WT MEFs, the last 3 lanes are Nrf1 KO MEFs. Lanes 1 and 4 were treated with DMSO for 4 hours. Lanes 2, 3, 5 and 6 were treated with 10 uM MG132 for 4 hours. (TCF11 is the name of the human homologue of mouse Nrf1). The upper band is the non-cleaved cytoplasmic form of Nrf1. The lower band is the cleaved nuclear form.
Antibody information
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: TCF11/NRF1
Catalog number: 8052
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Gly129 of human TCF11 protein. Reacts with human, mouse, and monkey by western blot.
Validation Method
Western blot
Sample
WT and Nrf1-KO MEFs.
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