This is a knockout-validated antibody summary, based on the publications "T cell factor 1 regulates thymocyte survival via a RORγt-dependent pathway", as cited below [1], and "TCF7 is not essential for glucose homeostasis in mice" for western blot knockout validation (figure s7a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of TCF-1 protein expression in wild-type (WT), RORγt −/− and TCF-1 −/− mouse thymocytes. β-actin expression served as a loading control. From figure 1 [1].

Company: Cell Signaling Technology

Antibody: TCF

Catalog number: 2203S

Summary: Rabbit IgG monoclonal antibody raised against a synthetic peptide corresponding to a region surrounding Pro95 of human TCF1 protein. It detects endogenous levels of total TCF1 protein of human and mouse origin by western blot, immunoprecipitation, immunohistochemistry (paraffin), immunocytochemistry and flow cytometry. This antibody does not recognize the dominant negative isoforms of TCF1 lacking the amino-terminal β-catenin binding domain and does not cross-react with LEF1.

Western blot.

Thymocytes isolated from mice (lysed in lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 4 mM KCl, 1 mM MgCl2, 1 mM Na3VO4, 10% glycerol, 1% NP-40, and protease inhibitors))

Membranes were blocked in blocking buffer (LI-COR)

primary antibody for 1 hour to overnight at 4°C

detected by an Odyssey Infrared Imaging System (LI-COR)