This is a knockout-validated antibody summary, based on the publication "Minimized human telomerase maintains telomeres and resolves endogenous roles of H/ACA proteins, TCAB1, and Cajal bodies", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Novus
Antibody: TCAB1
Catalog number: NB100-68252
Summary: Rabbit polyclonal against an epitope mapping to a region between residue 1 and 50 of human WD repeat. Reacts with human and monkey by western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and ChIP.
Western blot | Immunocytochemistry
TCAB1 and Coilin in clonal KO HCT116 cells (with LhTRmin + TERT overexpression).
WB: 4% nonfat milk (Carnation) in TBS buffer (150 mM NaCl, 50 mM Tris pH 7.5).
ICC: 4% BSA in PBS for 1 hr at room temperature or overnight at 4°C.
WB: 1:2,000 dilution in blocking buffer at 4°C overnight.
ICC: 1:300 in blocking buffer for 1 hr at room temperature or overnight at 4°C.
WB: 1:10,000 diluted goat anti-rabbit IR Dye 800 (Rockland Immunochemicals, Pottstown, PA) in blocking buffer buffer for 1 hr at room temperature.
ICC: Alexa Fluor 568 (Thermo) secondary antibodies diluted in blocking buffer for 1 hr at room temperature.
WB: blots were scanned using a LI-COR Odyssey imager.
ICC: images were acquired using a Zeiss LSM510 Meta confocal microscope with a ×100/1.49 Apo objective, with 364-, 488-, and/or 543-nm laser excitation.
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- geneproduct