TC-PTP antibody | knockout validation | R&D Systems MAB1930

This is a knockout-validated antibody summary, based on the publication "Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TC-PTP antibody | knockout validation | R&D Systems MAB1930 figure 1
Figure 1. Western blot analysis of TC-PTP in the epidermis from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. Tubulin was used as loading control. From [1].
Antibody information

Mouse monoclonal IgG2a

Company: R&D Systems

Antibody: TC-PTP

Catalog number: MAB1930

Summary: Mouse monoclonal IgG2a against recombinant human TC-PTP Pro2-Asn314. Reacts with human, mouse and rat by western blot.

Validation Method

Western blot

Sample

Epidermis from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. Tissues were lysed with RIPA buffer (Thermo Fisher Scientific) containing 1% Triton X-100, protease inhibitor cocktail (Sigma-Aldrich) and phosphatase inhibitor cocktail I, II (Sigma-Aldrich).

Primary incubation

Overnight at 4 °C.

Secondary incubation

Secondary antibody conjugated to horseradish peroxidase.

Detection

Enhanced chemiluminescence detection reagents (Amersham Biosciences).

References
  1. Lee H, Kim M, Baek M, Morales L, Jang I, Slaga T, et al. Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development.. Sci Rep. 2017;7:45077 pubmed publisher