This is a knockout-validated antibody summary, based on the publication "Lack of Genetic Interaction between Tbx18 and Tbx2/Tbx20 in Mouse Epicardial Development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunofluorescence analysis of TBX18 on transverse E10.5 sections through right ventricles of control and Tbx18cre/+;Tbx2fl/fl;R26mTmG/+ mice. Pericardial cells are positive for TBX2 in the control but not in Tbx18cre/+;Tbx2fl/fl;R26mTmG/+ mice. Scale bars are 50 μm. From [1].

Rabbit polyclonal IgG

Company: Millipore

Antibody: TBX2

Catalog number: 07-318

Summary: Rabbit polyclonal IgG against a synthetic protein corresponding to residues 554-702 of human TBX2. Reacts with human and mouse by western blot and immunoprecipitation.

Immunohistochemistry

Heart sections of of control and Tbx18cre/+;Tbx2fl/fl;R26mTmG/+ mice embryos. Embryos were fixed in 4% paraformaldehyde overnight and paraffin embedded for sectioning.

1:1,000 dilution. Primary antibody was amplified using the Tyramide Signal Amplification (TSA) system from Perkin-Elmer (NEL702001KT, Perkin Elmer).

1:200 dilution Alexa488-conjugated anti-rabbit IgG (Invitrogen).

Leica DM5000 microscope with Leica DFC300FX digital camera.