This is a knockout-validated antibody summary, based on the publication "IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1 (kappa light chain)

Company: Imgenex, now part of Bio-techne.

Antibody: TAp73

Catalog number: IMG-246, the same as NBP2-24737

Summary: Mouse monoclonal IgG1 (kappa light chain) raised against full-length human p73. Reacts with human, mouse and rat protein in western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, ChIP and flow cytometry.

Western blot

Mouse embryo fibroblasts (MEFs) of WT; ΔNp73−/−; and p73−/− genotypes. Thymic lymphomas of p53−/−; p53−/−, ΔNp63+/− and ΔNp73−/−;p53−/− mice and p73−/− MEFs.

1:500 dilution.

Horseradish peroxidase-conjugated antibodies at 1:5,000 dilution (Jackson Lab).

Using the ECL Plus Kit (Amersham) following the manufacturer’s protocol and x-ray autoradiography.

Please see Extended Data Figure 1d and 2e in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.