This is a knockout-validated antibody summary, based on the publication "Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: TAX1BP1
Catalog number: 5105
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy terminus of human TAX1BP1 protein. Reacts with human by western blot and immunoprecipitation.
Western blot
Control and TAX1BP1 knockout H4 cell lines. Cells were lysed in RIPA buffer (#9806, Cell Signaling Technology) supplemented with sodium dodecyl sulfate (SDS, Boston BioProducts) to 1% final concentration, and protease inhibitor tablets (Complete EDTA-free, Roche).
5% non-fat dry milk (Bio-Rad) in TBS with 0.2% Tween-20 (Boston BioProducts).
1:1,000 dilution in 5% bovine serum albumin (BSA, Cell Signaling Technology) in TBS with 0.2% Tween-20 at 4°C overnight.
1:10,000 dilution HRP-conjugated secondary antibodies diluted in blocking solution at room temperature for 1 h.
Pico or West Femto SuperSignal ECL reagents (Pierce) and film (GE Healthcare).
Immunoblot analysis of control or clonal TAX1BP1 knockout H4 cell lines blotted with the indicated antibodies. Please see Figure 3j in the article [1].
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