This is a knockout-validated antibody summary, based on the publication "TRAF Family Member-associated NF-κB Activator (TANK) Inhibits Genotoxic Nuclear Factor κB Activation by Facilitating Deubiquitinase USP10-dependent Deubiquitination of TRAF6 Ligase", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of TANK protein in wild-type and TANK-KO HEK293T cells. Tubulin was used as loading control. From [1].

Rabbit polyclonal

Company: Cell Signaling

Antibody: TANK

Catalog number: 2141

Summary: Rabbit polyclonal against a synthetic peptide corresponding to residues surrounding Ser357 of human TANK. Reacts with human, mouse, and rat by western blot and immunoprecipitation. It is predicted to react based on 100% sequence homology with monkey, bovine, and dog.

Western blot

Wild-type and TANK knockout HEK293T cells.

ECL exposure.

USP10 and MCPIP1 antibodies were also KO validated (Fig 4 and 3, respectively).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.