TAK1 antibody | knockout validation | Cell Signaling 5206

This is a knockout-validated antibody summary, based on the publication "Epigenetic Silencing of miR-33b Promotes Peritoneal Metastases of Ovarian Cancer by Modulating the TAK1/FASN/CPT1A/NF-κB Axis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TAK1 antibody | knockout validation | Cell Signaling 5206 figure 1
Figure 1. TAK1 knockout in ES-2 and MES-OV cells was confirmed by western blotting assay. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: TAK1

Catalog number: 5206

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Gln600 of human TAK1 protein. React with human, mouse and rat. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and TAK1-KO ES-2 and MES-OV cells.

Primary incubation

1:1,000 dilution overnight at 4 °C.

Secondary incubation

1:15,000 dilution fluorescence-conjugated secondary antibodies (LI-COR IRDye 680/800 CW).

Detection

LI-COR Odyssey CLx Imager (Lincoln, NE, USA).

References
  1. Wang X, Yung M, Sharma R, Chen F, Poon Y, Lam W, et al. Epigenetic Silencing of miR-33b Promotes Peritoneal Metastases of Ovarian Cancer by Modulating the TAK1/FASN/CPT1A/NF-κB Axis. Cancers (Basel). 2021;13: pubmed publisher