TAG1 antibody | knockout validation | DHSB 4D7/TAG1

This is a knockout-validated antibody summary, based on the publication "TAG-1 Multifunctionality Coordinates Neuronal Migration, Axon Guidance, and Fasciculation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TAG1 antibody | knockout validation | DHSB 4D7/TAG1 figure 1
Figure 1. TAG-1/TUJ1/Hoechst immunolabeling of E9.5 spinal cord sections from TAG-1cKO/cKO and Olig2Cre/+; TAG-1cKO/cKO embryos shows loss of TAG-1 from cells in the ventral horn and TUJ1+ motor axons in VRs (arrows) of Olig2Cre/+; TAG-1cKO/cKO embryos, but not TAG-1cKO/cKO littermates. From [1].
Antibody information

Mouse monoclonal IgM

Company: DHSB

Antibody: TAG1

Catalog number: 4D7/TAG1

Summary: Mouse monoclonal IgM against (fetal) rat brain lysate. Reacts with mouse and rat. Suitable for western blot, function blocking, immunohistochemistry, immunoprecipitation.

Validation Method

Immunohistochemistry

Sample

Control and TAG-1-KO spinal cord tissue sections.

Primary incubation

1:200 dilution.

Secondary incubation

1:200 dilution Alexa-conjugated donkey anti-mouse (Invitrogen).

Detection

Nikon Eclipse Ti inverted microscope with Andor CCD camera or an Olympus FV3000 confocal laser scanning microscope.

References
  1. Suter T, Blagburn S, Fisher S, Anderson Keightly H, D Elia K, Jaworski A. TAG-1 Multifunctionality Coordinates Neuronal Migration, Axon Guidance, and Fasciculation. Cell Rep. 2020;30:1164-1177.e7 pubmed publisher