TACO1 antibody | knockout validation | Proteintech 21147-1-AP
DOI
//dx.doi.org/10.13070/ko.en.7.2203
Date
2017-03-18

This is a knockout-validated antibody summary, based on the publication "Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TACO1 antibody | knockout validation | Proteintech 21147-1-AP figure 1
Figure 1. TACO1 protein levels were determined in mitochondria isolated from livers and hearts of Taco1wt/wt and Taco1mut/mut mice by immunoblotting. Porin was used as a loading control. The data are representative of results obtained from at least 8 mice from each strain. From [1].
Antibody information

Rabbit polyclonal IgG

Company: Proteintech

Antibody: TACO1

Catalog number: 21147-1-AP

Summary: Rabbit polyclonal IgG against TACO1/CCDC44 fusion protein. Reacts with human, mouse, and rat. Suitable for western blot, immunoprecipitation, immunohistochemistry and ELISA.

Validation Method

Western blot

Sample

Mitochondria isolated from livers and hearts of Taco1wt/wt and Taco1mut/mut (causing loss of the TACO1 protein) mice.

Primary incubation

Diluted in Odyssey Blocking Buffer (Li-Cor).

Secondary incubation

IR Dye 800CW goat anti-rabbit IgG secondary antibodies.

Detection

Odyssey Infrared Imaging System (Li-Cor).

References
  1. Richman T, Spahr H, Ermer J, Davies S, Viola H, Bates K, et al. Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice. Nat Commun. 2016;7:11884 pubmed publisher