TAB3 antibody | knockout validation | Cell Signaling 14241

This is a knockout-validated antibody summary, based on the publication "Interleukin-1 and TRAF6-dependent activation of TAK1 in the absence of TAB2 and TAB3", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TAB3 antibody | knockout validation | Cell Signaling 14241 figure 1
Figure 1. Generation of IL-1R* cells lacking TAB2 and TAB3 or all three TAB subunits. TAK1 was immunoprecipitated from the extracts of WT IL-1R* cells (lane 1), two different clones (4 and 11) of cells devoid of TAB2 and TAB3 (lanes 2 and 3) and two different clones (A4 and H17) lacking expression of all three TAB components. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: TAB3

Catalog number: 14241

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Arg375 of human TAB3 protein. Reacts with human, mouse, and rat by western blot.

Validation Method

Western blot

Sample

WT, TAB2/3-DKO and TAB1-3-TKO IL-1R* cells.

Secondary incubation

Secondary antibodies coupled to horseradish peroxidase (Thermo Scientific).

Detection

ECL detection system (GE Healthcare).

References
  1. Zhang J, MacArtney T, Peggie M, Cohen P. Interleukin-1 and TRAF6-dependent activation of TAK1 in the absence of TAB2 and TAB3.. Biochem J. 2017;474:2235-2248 pubmed publisher