This is a knockout-validated antibody summary, based on the publication "NK cells regulate CXCR2+ neutrophil recruitment during acute lung injury", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz Biotechnology

Antibody: T-bet

Catalog number: sc-21749

Summary: Mouse monoclonal IgG1 (kappa light chain) against full length recombinant murine T-bet. Recommended for detection of T-bet of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, flow cytometry and ELISA.

Western blot

Lung tissue from wild-type and Tbet-/- mice. Tissues were disrupted in T-Per Tissue Protein Extraction Reagent with Pierce Phosphatase and Protease Inhibitor (Thermo Fisher Scientific, Rockford, IL, USA).

5% blotting-grade nonfat milk (Bio-Rad) in TBST for 1 h at room temperature.

1:100 dilution overnight at 4°C.

1:10,000 dilution goat anti-mouse secondary antibody (31172; Thermo Fisher Scientific) for 1 h at room temperature.

Enhanced chemiluminescence (Thermo Fisher Scientific).

Immunoblot of Tbet protein in whole-lung tissues on day 0, 1, 3, and 5 after LPS treatment. Please see Figure 1b in the article [1].

The same clone (4B10) is sold as BioLegend 644811, 644812, 644815, 644816, 644823, 644824, 644801, 644802, 644803, 644804, 644805, 644806, 644807, 644808, 644809, 644810, 644813, 644814, 644817, 644819, 644825, 644827, 644828, 644829, 644830, 644831; Santa Cruz Biotechnology sc-21749, sc-21749 AC, sc-21749 X; Bio X Cell BE0100, BE0100-5MG; BD Biosciences 561265, 561262, 561264.