Syntaxin 18 antibody | knockout validation | Santa Cruz sc-293067

This is a knockout-validated antibody summary, based on the publication "Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Syntaxin 18 antibody | knockout validation | Santa Cruz sc-293067 figure 1
Figure 1. Western blot of the Stx18 in control and Stx18-KO 3T3-L1 preadipocytes. From [1].
Antibody information

Mouse monoclonal IgG2b (kappa light chain)

Company: Santa Cruz

Antibody: Syntaxin 18

Catalog number: sc-293067

Summary: Mouse monoclonal IgG2b (kappa light chain) against amino acids 220-331 of rat Syntaxin 18. Recommended for detection of Syntaxin 18 of mouse, rat, human and canine origin by western blot, immunoprecipitation and immunofluorescence.

Validation Method

Western blot

Sample

Control and Stx18-KO 3T3-L1 preadipocytes.

Secondary incubation

HRP-conjugated goat anti–mouse IgG (GE Healthcare).

References
  1. Xu D, Li Y, Wu L, Li Y, Zhao D, Yu J, et al. Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions.. J Cell Biol. 2018;0: pubmed publisher