This is a knockout-validated antibody summary, based on the publication "Synaptotagmin 7 confers frequency invariance onto specialized depressing synapses", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. High-power images of the deep cerebellar nuclei (DCN) immunolabeled for Calbindin and Syt7 shown for young (c1, P13-15), juvenile (c2, P21-32), and adult (c3, P80-110) wildtype and Syt7 knockouts. Scale bar, 10 μm. From [1].

Mouse monoclonal IgG2b

Company: UC Davis/NIH NeuroMab Facility

Antibody: Synaptotagmin-7

Catalog number: 75-265

Summary: Mouse monoclonal IgG2b against a fusion protein amino acids 150-239 (cytoplasmic C2A domain) of mouse

Synaptotagmin-7. Reacts with mouse and rat. Suitable for immunoblot, immunocytochemistry and immunohistochemistry.

Western blot

WT and Syt7 knockout mice brains. Tissues were removed and post-fixed in PFA overnight before sectioning.

4% Normal Goat Serum in 0.1 % triton X-100 for 1 hr at room temperature.

1:100 dilution (1 μg/mL) overnight at 4°C.

Anti-Mouse-AlexaFluor647 (Abcam ab150115) for 2 hrs at room temperature.

Olympus Fluoview1000 confocal microscope.

The same clone (N275/14) is sold as Neuromab 73-265, 75-265; EMD Millipore MABN665.