This is a knockout-validated antibody summary, based on the publication "Synaptotagmin 2 (Syt2) Drives Fast Release Redundantly with Syt1 at the Output Synapses of Parvalbumin-Expressing Inhibitory Neurons", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunohistochemistry of Syt1+/+ mouse (control) and a littermate P15 Syt1lox/lox mouse injected with lenti:oChIEFeYFP-IRES-Cre into the MNTB at P1 (thus producing Syt1 cKO synapses). Note the presence (upper panels) and absence (lower panels) of weak perisomatic Syt1 signal nerve terminals positive for VGAT and oChIEFeYFP (arrowheads). From [1].

Mouse monoclonal IgG2a (κ light chain)

Company: Synaptic Systems

Antibody: Synaptotagmin 1

Catalog number: 105 011

Summary: Mouse monoclonal IgG2a (κ light chain) against recombinant protein corresponding to AA 80 to 421 from rat Synaptotagmin 1. Reacts with human, rat, mouse, mammals, and zebrafish. Suitable for western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry (paraffin), electron microscopy and ELISA.

Immunohistochemistry

Brains from mice Syt1+/+ and Syt1 cKO at the synapses. Frozen brains were cut on a Hyrax S30 sliding microtome (Carl Zeiss) and processed for immunohistochemistry.

1:200 dilution overnight at 4°C.

1:200 dilution anti-mouse Alexa-647 (A31571, Thermo Fisher Scientific) for 1 h at room temperature.

Confocal images were acquired with an upright LSM 700 microscope (Carl Zeiss) equipped with 488, 563, and 633 nm laser lines and a Plan-Apochromat 40/1.30 NA oil-immersion objective (pixel size was 90 nm).