This is a knockout-validated antibody summary, based on the publication "Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Representative immunoblots and quantification of the indicated proteins in homogenates of hippocampus from n=4 21-day-old male mice for each genotype. From [1].

Mouse monoclonal IgG1κ

Company: Millipore

Antibody: Synaptophysin

Catalog number: MAB5258

Summary: Mouse monoclonal IgG1κ against vesicular fraction of bovine brain. The SY38 epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus & Betz 1990). Reacts with avian, bovine, fish, human, mouse, and rat. Suitable for western blotting, immunocytochemistry, immunohistochemistry and flow cytometry.

Western blot

Hippocampus from WT and Synaptophysin 1/2-Synaptogyrin 1/3 -QKO mice.

1:2,000 dilution.