This is a knockout-validated antibody summary, based on the publication "Synergistic actions of v-SNARE transmembrane domains and membrane-curvature modifying lipids in neurotransmitter release", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Exemplary immunofluorescence images of wt and dko cells expressing either GFP, VAMP2 or its TMD mutant variants. Cells were immunolabeled with an affinity purified mouse monoclonal antibody against VAMP2 (clone 69.1, antigen epitope amino acid position 1–14). Note that signals in dko cells expressing VAMP2 or its mutant variants were stronger than in wt+GFP cells and were visualized after adjustment of the camera’s exposure time (e.g. wt+GFP,1.18s; dko+VAMP2, 0.076 s). From [1].

Mouse monoclonal IgG1 (κ light chain)

Company: Synaptic Systems

Antibody: 104 211

Catalog number: Synaptobrevin 2

Summary: Mouse monoclonal IgG1 (κ light chain) against a synthetic peptide corresponding to aa 2 to 17 from rat Synaptobrevin2. Reacts with human, rat, mouse and hamster. Suitable for western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, immunohistochemistry (paraffin), electron microscopy and ELISA.

Immunocytochemistry

WT and VAMP2/VAMP3 double knock-out mouse chromaffin cells.

Digital images (eight bit encoded) were acquired with an AxioCam MRm-CCD camera (Carl Zeiss, Inc) on the stage a Zeiss AxioVert 25 microscope using a 100x fluar oil-immersion objective (NA, 1.3, Zeiss, Göttingen, Germany).