Sur-8 antibody | knockout validation | Santa Cruz sc-514779

This is a knockout-validated antibody summary, based on the publication "Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Sur-8 antibody | knockout validation | Santa Cruz sc-514779 figure 1
Figure 1. Western blot for SHOC2 expression in clonal knockout cells derived from PC9-Cas9-RIT1M90I cells. Vinculin was used as a loading control. From [1].
Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: Sur-8

Catalog number: sc-514779

Summary: Mouse monoclonal antibody against amino acids 5-304 mapping near the N-terminus of human Sur-8 (SHOC2). Recommended for detection of Sur-8 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, and ELISA.

Validation Method

Western blot

Sample

Control and SHOC2-KO PC9 cells.

Blocking agent

Tris Buffered Saline (TBS) with 1% Casein (BioRad) for 1h at room temperature.

Primary incubation

1:100 dilution in blocking buffer overnight at 4°C.

Secondary incubation

StarBright (BioRad) or IRDye (LiCOR) secondary antibodies.

Detection

ChemiDoc MP Imaging System (BioRad).

References
  1. Vichas A, Riley A, Nkinsi N, Kamlapurkar S, Parrish P, Lo A, et al. Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer. Nat Commun. 2021;12:4789 pubmed publisher