This is a knockout-validated antibody summary, based on the publication "A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot of SOD1 protein levels in control sgRNA and SOD1-KO SH-SY5Y cells normalized to input GAPDH loading control. Courtesy from the Authors [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Superoxide Dismutase 1

Catalog number: ab79390

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human Superoxide Dismutase 1 (aa 50-150). Reacts with human by western blot.

Western blot

Control and SOD1-KO SH-SY5Y cells.

5% dry fat-free milk in TBST (Tris Buffered Saline with Tween 20).

For 1 h at room temperature or overnight at 4 C in 2% dry fat-free milk in TBST.

HRP-conjugated secondary Ab for 1 h in 2% dry fat-free milk in TBST.

Chemiluminescence (Thermo Fisher Scientific) and imaged using a molecular imaging station (Kodak ISM 2000MM).