This is a knockout-validated antibody summary, based on the publication "Effects of Stathmin 1 Gene Knockout on Behaviors and Dopaminergic Markers in Mice Exposed to Social Defeat Stress", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative Immunohistochemistry staining images of anti-stathmin 1 (red) in the prefrontal cortex (PFC), dorsal hippocampus (HIP), amygdala (AMY) and dorsal striatum (dST) of WT control. No signal was detected in the KO control in all regions. Scale bar, 50 µm. From [1].

Rabbit monoclonal Ig

Company: Abcam

Antibody: Stathmin 1

Catalog number: ab52630

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Stathmin 1 (aa 100 to the C-terminus, C terminal). Reacts with mouse, rat, and human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, flow cytometry and immunohistochemistry (paraffin).

Immunohistochemistry

WT and STMN1-KO mouse brain. Free-floating tissue sections.

Peroxidase-blocking solution (S2023, Dako, Glostrup, Denmark) and incubated in protein-blocking solution (X0909, Dako).

1:200 dilution.

1:200 dilution biotinylated secondary antibodies (E0466; Dako).

1:200 dilution streptavidin-horseradish peroxidase solution (P0397; Dako) and incubated with the chromogen AEC (3-amino-9-ethylcarbazole, K3461, Dako).