This is a knockout-validated antibody summary, based on the publication "YAP-independent mechanotransduction drives breast cancer progression", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of MCF10A::Cas9/sgGAL4 or MCF10A::Cas9/sgSTAT3 cells with or without 2 μg/ml dox for 72 h and probed for STAT3. p38 was used as a loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: Stat3

Catalog number: sc-8019

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 50-240 mapping at the N-terminus of human Stat3. Recommended for detection of Stat3 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including and equine, bovine and porcine.

Western blot

Control and STAT3 KO MCF10A cells.

5% milk in TBS-T (137 mM NaCl, 2.7 mM KCl, 19 mM Tris base, 0.1% Tween, pH 7.4)

1:500 dilution.

1:10,000 dilution IRDye 680 or 800-conjugated secondary antibodies (LI-COR Biotechnology) for 1 h.

Li-COR Odyssey imaging system (Li-COR Biotechnology).