Stat3 antibody | knockout validation | BD Biosciences 610189
DOI
//dx.doi.org/10.13070/ko.en.7.2246
Date
2017-04-08

This is a knockout-validated antibody summary, based on the publication "STAT3 Inhibition Enhances the Therapeutic Efficacy of Immunogenic Chemotherapy by Stimulating Type 1 Interferon Production by Cancer Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: Stat3

Catalog number: 610189

Summary: Mouse monoclonal IgG1 against rat Stat3 (aa. 1-175). Reacts with rat, human, mouse, dog, chicken, and frog. Suitable for western blot and immunoprecipitation. Not recommended for immunofluorescence or immunohistochemistry.

Validation Method

Western blot

Sample

WT and Stat3-KO MCA205 fibrosarcomas and CT26 colorectal carcinoma cells. Cells were lysed with lysis buffer containing cOmplete protease inhibitor cocktail; Roche.

Blocking agent

5% non-fat milk in 1× TBS containing 0.1% Tween-20.

Primary incubation

1:500 dilution overnight.

Secondary incubation

1:5,000 dilution HRP-conjugated secondary antibodies (SouthernBiotech).

Detection

ECL Western Blotting Detection Reagent (Amersham, GE Healthcare) using an ImageQuant LAS 4000 (GE Healthcare).

Figure

Knockout of the Stat3 gene with ZFN technology was validated by immunoblots in MCA205 (left) and CT26 (right) cells. Actin served as loading control. Please see Figure 2a in the article [1].

References
  1. Yang H, Yamazaki T, Pietrocola F, Zhou H, Zitvogel L, Ma Y, et al. STAT3 Inhibition Enhances the Therapeutic Efficacy of Immunogenic Chemotherapy by Stimulating Type 1 Interferon Production by Cancer Cells. Cancer Res. 2015;75:3812-22 pubmed publisher