Stat1 antibody | knockout validation | Cell Signaling 14994

This is a knockout-validated antibody summary, based on publications "IFN/STAT signaling controls tumorigenesis and the drug response in colorectal cancer", as cited below [1], and "cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS model" for western blot knockout validation (figure s4a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Stat1 antibody | knockout validation | Cell Signaling 14994 figure 1
Figure 1. Immunohistochemical analysis of intestinal tumors. Apcfl/fl, Lgr5‐CreERT2, Stat1wt/wt (Stat1wt/wt) or Apcfl/fl, Lgr5‐CreERT2, Stat1null/null (Stat1 null/null) mice were analyzed 26 days after administration of 4OHT. Immunohistochemical analysis is shown using an anti‐Stat1 antibody. Bar = 200 μm. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Stat1

Catalog number: 14994

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Pro688 of human Stat1 protein. Reacts with human, monkey, mouse, and rat. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry, flow cytometry and chromatin IP.

Stat1 antibody | knockout validation | Cell Signaling 14994 figure 2
Figure 2. Immunofluorescence analysis of STAT1 knockout and control organoids stained with anti STAT1 antibody. From [1].
Validation Method

Western blot | Immunohistochemistry | Immunocytochemistry

Sample

Intestinal tumors and organoid from control and Stat1-KO cells.

Stat1 antibody | knockout validation | Cell Signaling 14994 figure 3
Figure 3. Complete loss of STAT1 protein was confirmed by immunoblot analysis. From [1].
Primary incubation

IHC: 1:300 dilution.

Secondary incubation

IHC: Envision+ system‐HRP labeled polymer (Dako) and 3,3′‐diaminobenzidine (Vector, Burlingame, CA, USA) according to the manufacturer's instructions.

References
  1. Sakahara M, Okamoto T, Oyanagi J, Takano H, Natsume Y, Yamanaka H, et al. IFN/STAT signaling controls tumorigenesis and the drug response in colorectal cancer. Cancer Sci. 2019;110:1293-1305 pubmed publisher
  2. Tan H, Yong Y, Xue Y, Liu H, Furihata T, Shankar E, et al. cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS model. iScience. 2022;25:104404 pubmed publisher